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KMID : 0613820130230080989
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Journal of Life Science
2013 Volume.23 No. 8 p.989 ~ p.997
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Cytoprotective Effects and Gene Expression Patterns Observed Based on the Antioxidant Activity of Lonicera japonica Extract
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Cho Won-June
Yoon Hee-Seung
Kim Yong-Hyun
Kim Jung-Min
Yoo Il-Jae
Han Man-Deuk
Bang In-Seok
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Abstract
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In this study, based on the antioxidative effects in organic solvent fractions obtained from the main methanolic extract of L. japonica, the protective cellular effects and gene expression patterns of ethyl acetate fractions on H₂O₂-induced Raw 264.7 cell death (IC_50) were analyzed. The antioxidant activity of the fractions measured using DPPH free radical scavenging activity increased in a dose-dependent manner, and the ED_50 exhibited the highest 39.56μg/ml in the ethyl acetate fraction. In addition, the ethyl acetate fractions' cell viability on H₂O₂-induced Raw 264.7 cell damage increased in a concentration-dependent manner, showed a visible cell survival rate of 82.49% at a concentration of 100μg/ml. The gene expression patterns related to the ethyl acetate fractions' cytoprotective effect in H₂O₂-induced Raw 264.7 cell damage presented similar patterns to those of BHA. In comparative analysis for antioxidant activity-related genes affected by ethyl acetate fractions and BHA in H₂O₂-induced Raw 264.7 cells, both ethyl acetate fractions and BHA showed very similar gene expression patterns, but the gene expression level of the heme oxygenase 1 (Hmox1) gene making antioxidant enzymes in cells was four times higher in ethyl acetate fractions than BHA. In inflammation-related genes in H₂O₂induced Raw 264.7 cells, the T-box transcription factor (Tbx21) gene was expressed about two times more frequently in the ethyl acetate fraction treatment group, while it was expressed half as frequently in the BHA treatment group.
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KEYWORD
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Lonicera japonica, antioxidant activity, inflammation, gene expression
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